Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
نویسندگان
چکیده
منابع مشابه
Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملInfluence of Zinc and Cadmium on Physiological and Biochemical Characteristics of Maize (Zea mays L.)
This research was carried out as pod cultivation to evaluate effect of different level of zinc and cadmium on agro physiological traits of Maize via factorial experiment based on completelly randomized design with three replications along 2012-2013. The factors included different level of Zinc (Zn1=0 or Control, Zn2=15 and Zn3=30 mg.kg-1) and Cadmium (Cd1=0 or control, Cd2=10, Cd3=20 and Cd4=30...
متن کاملInfluence of Temperature Stress on in Vitro Fertilization and Heat Shock Protein Synthesis in Maize (Zea mays L.) Reproductive Tissues.
This study was conducted to investigate the response of maize (Zea mays) male and female mature reproductive tissues to temperature stress. We have tested the fertilization abilities of the stressed spikelets and pollen using in vitro pollination-fertilization to determine their respective tolerance to stress. The synthesis of heat shock proteins (HSPs) was also analyzed in male and female tiss...
متن کاملInfluence of Explant Nodal Positions on the In Vitro Shoot Regeneration of Rose
The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...
متن کاملEffect of Different Sugars on Shoot Regeneration of Maize (zea Mays L.)
Effect of various concentrations of different sugars was investigated for induction of root and shoot from maize. The seedling development was induced with the application of fructose, maltose and sucrose at different concentrations (0.25%, 0.5%, 0.75%, 1.0%, 2.5%, 5%, and 10%) of each. Dissected embryos were transferred in 1⁄2 MS basal media fortified with various concentrations of different c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pakistan Journal of Agricultural Research
سال: 2020
ISSN: 0251-0480
DOI: 10.17582/journal.pjar/2020/33.4.917.925